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. 2003 Jun 19;551(Pt 2):455–466. doi: 10.1113/jphysiol.2003.042903

Figure 1. Specificity of the anti-sgk antibody and identification of sgk isoforms expressed in the kidney.

Figure 1

Western blots of proteins extracted from HEK 293 cells transfected with sgk1, sgk2 and sgk3 cDNAs, control (non-transfected cells), and renal tissue examined with anti-FLAG monoclonal and anti-sgk antibodies. The lane labelled kidney contains 5-fold more protein than the lanes loaded with HEK 293 cell lysates. The arrows on the left indicate molecular masses of standards.