Figure 6. Levels of expression of sgk1 analysed by Western blotting.

A, representative example of a Western blot. Equal amounts of proteins from renal cortex or medulla of rats subjected to various treatments to alter glucocorticoid and mineralocorticoid concentrations were examined by Western blotting with sgk antibody. B, quantification of the intensity of the sgk1 signal by scanning densitometry of gels (n = 4). Values were normalized to the intensity of the bands from normal rats. Lanes correspond to: (1) control rats, (2) low-salt diet, (3) adrenalectomy, (4) adrenalectomy and dexamethasone infusion by minipump, (5) single dose of aldosterone and (6) spironolactone, 3 h prior to harvesting kidneys. Values are reported as means ±s.d.* Significant statistical difference (Students's t test, P < 0.001).