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. 2003 Jun 24;551(Pt 2):491–501. doi: 10.1113/jphysiol.2003.045104

Table 1.

Primers used for real-time PCR amplification

Gene GenBank accession number Forward Primer (5′–3′) Reverse Primer (5′–3′) PCR product size (bp)
PGC-1α AF049330 AGTGTGCTGCTCTGGTTGGTG 613
GGAGGGTCATCGTTTGTGGTC
NRF-1 NM–010938 TTACTCTGCTGTGGCTGATGG 92
CCTCTGATGCTTGCGTCGTCT
NRF-2α M74515 AGGTGAGGAGATGGGCTGC 604
CGTTGTCCCCATTTTTGCG
MtTFA AJ312746 GAAAGCACAAATCAAGAGGAG 175
CTGCTTTTCATCATGAGACAG
COX I X14848 AGCAGGAATAGTAGGGACAGC 520
TGAGAGAAGTAGTAGGACGGC
COX IV X15029 TGGGAGTGTTGTGAAGAGTGA 273
GCAGTGAAGCCGATGAAGAAC
β-actin X00351 GGGTCAGAAGGATTCCTATG 238
GGTCTCAAACATGATCTGGG

PGC-1α, peroxisome proliferator activated receptor gamma co-activator 1α; NRF-1, nuclear respiratory factor 1; NRF-2α, nuclear respiratory factor 2 DNA-binding alpha subunit; mtTFA, mitochondrial transcription factor A; COX I and COX IV, cytochrome c oxidase subunits I and IV.