Table 1.
Gene | GenBank accession number | Forward Primer (5′–3′) Reverse Primer (5′–3′) | PCR product size (bp) |
---|---|---|---|
PGC-1α | AF049330 | AGTGTGCTGCTCTGGTTGGTG | 613 |
GGAGGGTCATCGTTTGTGGTC | |||
NRF-1 | NM–010938 | TTACTCTGCTGTGGCTGATGG | 92 |
CCTCTGATGCTTGCGTCGTCT | |||
NRF-2α | M74515 | AGGTGAGGAGATGGGCTGC | 604 |
CGTTGTCCCCATTTTTGCG | |||
MtTFA | AJ312746 | GAAAGCACAAATCAAGAGGAG | 175 |
CTGCTTTTCATCATGAGACAG | |||
COX I | X14848 | AGCAGGAATAGTAGGGACAGC | 520 |
TGAGAGAAGTAGTAGGACGGC | |||
COX IV | X15029 | TGGGAGTGTTGTGAAGAGTGA | 273 |
GCAGTGAAGCCGATGAAGAAC | |||
β-actin | X00351 | GGGTCAGAAGGATTCCTATG | 238 |
GGTCTCAAACATGATCTGGG |
PGC-1α, peroxisome proliferator activated receptor gamma co-activator 1α; NRF-1, nuclear respiratory factor 1; NRF-2α, nuclear respiratory factor 2 DNA-binding alpha subunit; mtTFA, mitochondrial transcription factor A; COX I and COX IV, cytochrome c oxidase subunits I and IV.