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. 2003 Jun 20;551(Pt 3):765–776. doi: 10.1113/jphysiol.2003.041707

Figure 5. The combined effect of buffering [Ca2+]i with BAPTA and inhibiting PKG activity with KT5823 on the response to Br-cGMP.

Figure 5

A and B, two representative cells illustrate the combined effect of the pretreatment of cells with KT5823 and BAPTA AM on the changes in CBF (black dots) and [Ca2+]i (grey lines) induced by 100 μM Br-cGMP (bar). KT5823 and BAPTA AM treatment prevented any significant increases in the [Ca2+]i and CBF in response to Br-cGMP. The basal CBF of the cells was 17.7 Hz (A) and 12.5 Hz (B). C and D, summaries of the combined effects of KT5823 and BAPTA AM on the initial (C) and sustained (D) increases in CBF induced by 100 μM Br-cGMP. The increase in the initial CBF (1.09 ± 0.03, n = 7) induced by Br-cGMP in the presence of KT5823 and BAPTA AM was significantly lower than the increase induced in cells without treatment (1.32 ± 0.08, n = 6, *P < 0.05). Similarly, the sustained increase in CBF (0.93 ± 0.03, n = 7) was significantly lower compared to cells without treatment (1.23 ± 0.05, n = 6, ***P < 0.001). The basal CBFs from each group were similar (12.4 ± 1.0 Hz, n = 6 and 15.3 ± 1.2 Hz, n = 7, P > 0.05).