Figure 1. Distribution of SK3 immunoreactivity in the urinary bladder wall.

A, SK3 immunoreactivity in a bladder cross-section from a control (C57BL6) mouse. Positive SK3 immunoreactivity (red) was seen in smooth muscle and urothelium. The inset shows a negative control, in which the tissue slice was pretreated with excess antigenic peptide. B, whole mount urothelial preparations stained for SK3 channels. Immunoreactivity is increased relative to control in SK3^T/T overexpressor urothelium, while DOX treatment suppresses SK3 immunostaining. C, whole mount smooth muscle preparations stained for SK3 channels obtained from control, SK3^T/T-overexpressing and SK3^T/T-suppressed mice.