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Ducts were pre-incubated with 10 μM BAPTA AM for 30 min. A, 1 μM ACh was applied to the BAPTA-loaded ducts (n = 4). B, the BAPTA-loaded ducts were first stimulated with 1 nM secretin and then 1 mM ethanol was added (n = 5). Values are means ± S.E.M.
