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. 2003 Jul 23;552(Pt 2):345–356. doi: 10.1113/jphysiol.2003.047167

Figure 6. Biochemical demonstration of tyrosine phosphorylation of CNGA1 and CNGB1 subunits.

Figure 6

A, Western blot analysis (anti-His6) of wild-type (WT; CNGA1 and CNGB1) and mutant (Y→F; CNGA1Y498F and CNGB1Y1097F) channel subunits. Before homogenization, oocytes expressing heteromeric CNG channels and non-injected oocytes (Control) were pretreated with pervanadate (PV), a PTP inhibitor, or lavendustin A (LavA), a PTK inhibitor. Molecular mass markers (in kDa) are shown on the right. B, autoradiograms of wild-type (CNGA1 and CNGB1) and mutant (CNGA1Y498F and CNGB1Y1097F) subunits.