Skip to main content
. 2003 Aug 22;552(Pt 3):823–832. doi: 10.1113/jphysiol.2003.049247

Figure 1. Transporter mRNA expression upon adaptation to carbohydrate diets.

Figure 1

Intestinal RNA samples were extracted from mucosal scrapping of the upper jejunum or of the ileum of wild-type (WT, filled bars) or GLUT-2 null (hatched bars) mice. Messenger abundance was quantified either by density scanning of Northern blots (GLUT2, SGLT1, GLUT5) or by RT-PCR in real time with a Light-Cycler (GLUT2, glucose 6-phosphatase (G6Pase)). Results were obtained with an average 6 mice fed low carbohydrate (LC, light grey), glucose-rich (HG, grey) or fructose-rich (HF, dark grey) diets for 5 days. All quantifications were normalized against L19 or the 18S ribosomal RNA. Results are expressed in arbitrary units ±s.e.m.; ** significant difference (P < 0.01) of data when compared with LC diet.