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. 2003 Sep 5;553(Pt 1):137–145. doi: 10.1113/jphysiol.2003.046268

Figure 2. Detection and quantification of renal BBM facilitative glucose transporters.

Figure 2

A, detection of GLUT1, GLUT2 and GLUT5 by Western blotting using BBM vesicles prepared from renal cortex of control (C), 2–4 week diabetic (D) and 2–4 week diabetic rats subjected to an overnight fast (FD). B, quantification of levels of GLUT1, GLUT2 and GLUT5 from bands produced by Western blotting of cortical BBM vesicles prepared from normal (open bars), 2–4 week diabetic (black bars) and overnight fasted, 2–4 week diabetic rats (grey bars). Results are expressed as mean ± s.e.m. Values were obtained from Western blots for each GLUT isoform carried out on six vesicle preparations. * P < 0.05, ** P < 0.001 compared to control; + P < 0.001 compared to 2–4 week diabetic.