Figure 3. Effect of titin-based passive tension on SL-dependent activation.

Data taken from Fig. 2 and Table 1. Lines are linear regression lines. Titin-based passive tension was obtained as described in Methods. ▵Titin-based passive tension, difference in titin-based passive tension at SL 1.9 and 2.4 µm. Passive tension data in Table 1 were used for analysis. Passive tension measured immediately after stretch is indicated as ‘high’. A, effect of titin-based passive tension on Ca²⁺ sensitivity (y = 0.037x + 0.003 (r² = 0.979, P < 0.01)). B, effect of titin-based passive tension on maximal Ca²⁺-activated tension (y = 2.857x − 0.294 (r² = 0.989, P < 0.005)).