Table 1.
The rates of MTSET modification in various cysteine mutants (s−1 M−1)
| Q164 | Q164C(−40mV) | Q164C/D172N | G168C | V169C | I171C | D172C | I176C | |
|---|---|---|---|---|---|---|---|---|
| Ctrl | 221 ± 46 (fast) | |||||||
| 22.0 ± 3.3 | 5.2 ± 0.6 | 63.5 ± 5.8 | 18.9 ± 2.4 | 15.7 ± 1.2 (slow) | 5.5 ± 0.5 | 42.4 ± 6.0 | 115.4 ± 1.8 | |
| (n = 12) | (n = 11) | (n = 7) | (n = 13) | (n = 8) | (n = 6) | (n = 5) | (n = 3) | |
| Spm | n.d | 7.7 ± 0.5* | 55.6 ± 6.8 | n.d | 12.8 ± 1.1 | 3.0 ± 0.5* | 65.6 ± 4.9* | 67.0 ± 5.3*** |
| (n = 5) | (n = 5) | (n = 6) | (n = 4) | (n = 4) | (n = 6) | (n =10) | (n = 3) |
Fast indicates the fast component and slow denotes the slow component of MTSET modification with two phases. Ctrl, experiment with control solution, Spin, experiment with spermine solution.
*
P < 0.05
***
P < 0.001, n.d. denotes not determined.