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The rates of MTSET modification in various cysteine mutants (s−1 m−1)
| V169C/D172N | I176C/D172N | D172C/E224G/E299S | I176C/D172N/E224G/E299S | |
|---|---|---|---|---|
| Ctrl | 8.2 ± 0.9 | 22.7 ± 2.3 | 50.5 ± 8.2 | 23.6 ± 1.5 |
| (n = 4) | (n = 3) | (n = 4) | (n = 7) | |
| Spm | 14.7 ± 1.4** | 33.8 ± 3.9* | 62.6 ± 8.3 | 24.1 ± 2.9 |
| (n = 6) | (n = 5) | (n = 5) | (n = 5) |
P < 0.05
P < 0.005.
