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. 2003 Oct 10;553(Pt 1):13–19. doi: 10.1113/jphysiol.2003.054551

Figure 2. Distribution of rSK1 protein in HEK 293 cells.

Figure 2

A, confocal image of HEK 293 cells transiently transfected with a YFP-tagged rSK1 construct (green filter) showing that the expressed protein is unevenly distributed around the cell and has a punctate staining pattern. B, the staining seen with rSK1-specific antibody UCL 56. Comparison with A shows that it recognizes the channel protein because the staining coincides well with YFP fluorescence. C, bright field image of cells depicted in A and B. D, overlay of images A, B and C. Scale bar is 20 µm.