Figure 3. AQP2 protein abundance in the kidney inner medulla of Nhe3+/+, Nhe3+/- and Nhe3−/− mice.
A, immunoblot of AQP2 protein level in the inner medulla. Both 29 and 35 kDa bands are expressed in the mouse kidney inner medulla. B, densitometry of AQP2 protein (29 kDa and 35 kDa bands) in the inner medulla showing a decrease in the abundance of both 29 kDa (n = 5) and 35 kDa (n = 5) bands in Nhe3−/− as compared to Nhe3+/+ mice. These two bands were not significantly altered in Nhe3+/- (n = 4) vs. Nhe3+/+ (n = 5); 3 µg of total protein from the inner medulla were loaded per lane.