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. 2003 Sep 18;553(Pt 2):511–522. doi: 10.1113/jphysiol.2003.053363

Figure 6. Northern hybridization of AQP2 mRNA in kidney medulla of Nhe3+/+ and Nhe3−/− mice.

Figure 6

Northern blots of AQP2 mRNA expression in whole medulla (outer + inner medulla) (A) and outer medulla (C). B and D are ratios of AQP2 mRNA to 28S rRNA for A and C, respectively. Results show a decrease (−54 %) in the expression of AQP2 mRNA in whole medulla of Nhe3−/− mice (B). In the outer medulla, the AQP2 mRNA expression was not altered in Nhe3−/− mice (D) vs. Nhe3+/+ mice. Each lane was loaded with 30 µg RNA isolated from pooled tissues harvested from two (whole medulla), or three mice (outer medulla).