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. 2003 Sep 18;553(Pt 2):511–522. doi: 10.1113/jphysiol.2003.053363

Figure 7. NKCC2 protein abundance and mRNA expression in Nhe3−/− and Nhe3+/+ mice.

Figure 7

A, immunoblot of NKCC2 protein level in the outer medulla harvested from Nhe3+/+, Nhe3+/- and Nhe3−/− mice. B, corresponding densitometric analysis showing 44 % decrease in the abundance of NKCC2 protein (n = 5) in Nhe3−/− as compared to Nhe3+/+ (n = 4) mice. NKCC2 protein was not significantly altered in Nhe3+/- (n = 4) as compared to Nhe3+/+ (n = 5); 12 µg of total protein from the outer medulla was loaded per lane. C, Northern hybridization of NKCC2 mRNA in whole medulla of Nhe3+/+ and Nhe3−/− mice. D, ratio of NKCC2 mRNA to 28S rRNA for Northern blot shown in C. The results show a significant decrease (−52 %) in the expression of NKCC2 mRNA in Nhe3−/− mice as compared to Nhe3+/+ mice (n = 3 for each); 30 µg RNA was loaded on each lane.