Table 2.
Effects of acetazolamide treatment for 72 h vs. its vehicle on AQP2 mRNA expression and total AQP2 protein abundance in the different regions of the mouse kidney
| AQP2 mRNA/28S rRNA | Total AQP2 protein abundance | |||||
|---|---|---|---|---|---|---|
| Vehicle | Acetazolamide | P | Vehicle | Acetazolamide | P | |
| Cortex | 100 ± 6 | 113 ± 7 | n.s. | 100 ± 3 | 80 ± 10 | n.s. |
| Outer medulla | n.d. | n.d. | — | 100 ± 8 | 56 ± 15 | 0.05 |
| Whole medulla | 100 ± 12 | 96 ± 4 | n.s. | n.d. | n.d. | — |
| Inner medulla | n.d. | n.d. | — | 100 ± 13 | 91 ± 19 | n.s. |
n.s., not significant; n.d., not determined. Total AQP2 protein = densitometry of 29 + 35 kDa bands. Results are means ± s.e.m. and are expressed as a percentage of vehicle (n = 4 or 5 mice in each group).