Table 1.

Effect of etsrp overexpression and knockdown on pu.1 expression at the 8- to 10-somite stages

	Normal or slightly distorted pu.1 pattern	Normal endogenous and ectopic pu.1 expression	Reduced or absent pu.1 expression	Reduced or absent endogenous and ectopic pu.1 expression
Control (n > 50)	100	0	0	0
etsrp MO (n = 40)	5 ± 5	0	95 ± 5	0
etsrp RNA (n = 57)	24 ± 11	16 ± 1	9 ± 9	51 ± 19
etsrpMO + RNA (n = 56)	1 ± 1	0	27 ± 1	72 ± 2

Etsrp RNA causes ectopic pu.1 expression in control embryos and etsrp morphants and also results in the reduction of endogenous etsrp expression. Results are percentage averages from 2 independent experiments.