Figure 2.

Hematopoietic reconstitution in mice injected with c-Myb^f/d or c-Myb^f/f bone marrow cells. Bone marrow cells (10⁶) from a c-Myb^f/f or a c-Myb^f/d mouse were injected in 3 lethally irradiated syngenic mice (11 Gy, 2 split doses of 5.5 Gy rad/each). After 3 months, the proportion of bone marrow cells expressing the Sca-1, c-Kit, Gr-1, and Mac-1 antigens was tested by immunostaining. Histograms show frequency of Sca-1⁺c-Kit⁻, Sca-1⁻c-Kit⁺, Sca-1⁺c-Kit⁺, Gr-1⁺, and Mac-1⁺ cells in the bone marrow of mice injected with c-Myb^f/f (A) or c-Myb^f/d (B) bone marrow cells. None of the differences in the values of histograms in panels A,B is statistically significant. As control, lethally irradiated mice not injected with bone marrow cells died after 2 weeks; bone marrow cells purified from mice injected with c-Myb^f/d cells were genotyped and showed the distinctive polymerase chain reaction (PCR) pattern of c-Myb^f/d donor cells.