Figure 1.
Hepatic extracellular matrix expression is differentially regulated in wild-type and SCID mice BALB/c and C57BL/6 mice. Liver fibrosis was induced by gavage with CCl4 (3.5 ml/kg, mixed 1:1 with corn oil administered at 7 day intervals). Seven days after the 4th dose of CCl4, animals were killed, and livers were fixed in 10% formalin and stained with Sirius Red as described. Collagen is stained by Sirius Red and appears black. (a) Normal control BALB/c receiving corn oil vehicle alone (identical to C57BL/6; data not shown). (b) Wild-type BALB/c. (c) Wild-type C57BL/6. (d) SCID BALB/c. (e) SCID C57BL/6. Representative photomicrographs are shown. (×90.)