Table 1.

Total hepatic collagen content after liver injury

Mouse strain	Control	Wild type	SCID
BALB/c	1.0  ±  0.05	2.98  ±  0.16*‡	2.21  ±  0.22*‡
C57BL/6	1.1  ±  0.06	1.52  ±  0.03*	2.12  ±  0.14*‡

Liver fibrosis was induced by gavage with CCl₄ (3.5 ml/kg, mixed 1:1 with corn oil, was administered at 7-day intervals) in wild-type and SCID BALB/c and C57BL/6 mice. Seven days after the 4th dose of CCl₄, animals were killed and livers fixed in 10% formalin. Liver tissue sections were stained with Sirius Red, and collagen was extracted and quantitated spectrophotometrically as described. Control mice received corn oil alone on the same schedule as animals receiving CCl₄. The value for normal BALB/c mice was arbitrarily set at 1; other values were normalized. 

^*P < 0.05 compared to control. 

^†P < 0.05 for BALB/c wild-type compared to C57BL/6 wild-type mice. 

^‡P < 0.05 compared to normal or wild type (n = 4 for each group; values shown are mean ± SD).