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Equilibrium dialysis studies of mirror image aptamers (see Fig. 2F). Fa, the fraction of active radiolabeled peptide bound to DNA, was determined, and curves were fitted as described in Materials and Methods. Radiolabeled peptide was either N-[3H]-acetyl-d-VP (filled symbols) or [3H]-l-VP (open symbols). (A) d-aptamer, no competitor peptide. (B) d-aptamer (10 μM), d-VP competitor. (C) l-aptamer, no competitor peptide. (D) l-aptamer (8 μM), l-VP or oxytocin competitor.
