Figure 6.

A 1.2-kb genomic fragment upstream of GRL1 functions as an inducible promoter element in vivo. (A) GFP1 reporter constructs. For a putative promoter, pH4.GFP1 utilizes the 300-bp histone H4-I promoter; pGRL1.GFP1 utilizes the 1200-bp genomic fragment upstream of GRL1; pΔH4.GFP1 utilizes a 300-bp fragment of the plasmid backbone (denoted by ∗). (B) Transformants bearing each of the three constructs were starved, stimulated, and followed during recovery. Total RNA was prepared at several time points, as in Fig. 4. Northern blot analyses of samples normalized for poly(A)⁺ RNA were probed with ³²P-labeled GFP1 cDNA. To the left of each blot is a diagram of the GFP1 locus designating each of the three reporter constructs. The size of the GFP1 mRNA is ≈900 bp.