Figure 3.

Gel shift assays of wild-type or mutant DNA fragments including the SRE-like sequences at −646, −395, and −287 bp. ³²P-labeled synthetic oligonucleotides (cav-646, cav-395, cav-287) were incubated in the absence of nuclear extract (lanes 1, 5, and 9), in the presence of nuclear extract (lanes 2, 6, and 10), with both nuclear extract and cold homologous DNA (lanes 3, 7, and 11) or with both nuclear extract and unlabeled mutant DNA (Δ1, Δ2, or Δ3 for cav-646, cav-395, or cav-287, respectively)(lanes 4, 8, and 12). The bound and unbound DNA fractions were resolved on polyacrylamide gels as described.