Figure 5.
Gel supershift assays of the essential SRE-like sequences at −646 and −395 bp in the presence of antibodies against SREBP-1 and Sp1. Nuclear extract was preincubated with 5 μg of anti-SREBP-1 or anti-Sp1 as described. Lanes: 1, free probe; 2, probe plus 4 μg nuclear extract; 3, probe plus nuclear extract plus cold DNA; 4, probe plus nuclear extract plus anti-SREBP-1; 5, probe plus nuclear extract plus anti-Sp1.