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Concentration dependence of the pseudo-first-order rate constant for the first phase of association of XTP with the Asp-449 → Asn mutant of the NG fragment of FtsY. The fitted hyperbola corresponds to an apparent Kd value of 21.3 ± 3.2 μM and a maximal rate of 135.9 ± 6.1 s−1. Conditions as in Fig. 3.
