Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Sep 30;94(20):10717–10722. doi: 10.1073/pnas.94.20.10717

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 1997, The National Academy of Sciences of the USA

PMC Copyright notice

CLARP is devoid of significant caspase activity when expressed in cells. (A) Flag-tagged CLARP was detected with anti-Flag antibody in lysates from 293T cells transiently transfected with pcDNA3 (lane 1) and pcDNA3-Flag-CLARP (lane 2). Molecular weight of CLARP was calculated from comparison with protein markers. Protein immunoprecipitates with anti-Flag antibody from cells transfected with pcDNA3-CLARP-Flag were incubated with the fluorogenic DEVD-AMC (B) or acetyl-Tyr-Val-Ala-Asp-AMC (YVAD-AMC) (C). Fluorescence was detected after 1 hr and 10 hr of incubation with CLARP complexes. Protein immunoprecipitates with anti-Flag antibody from cells transfected with pCDNA-CED-3-Flag were used as a positive control for the DEVD-AMC cleavage assay. For the YVAD enzymatic, whole Nonidet P-40 extract from cells transfected with pCDNA-caspase-8-AU1 was used as a positive control.