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Biological assay for hG-CSF. Serum neutrophil counts after treatment with rhG-CSF. Mice in the standard and the sample groups were treated with different doses of rhG-CSF (4, 12, and 36 μg/0.5 ml/mouse). The control group was treated with vehicle (0.1% BSA in PBS). The asterisks represent significant differences in comparison to the control group (** P<0.001). N = 6 mice per group.
