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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2007 Oct 9;40(4):700–709. doi: 10.1016/j.biocel.2007.10.002

Figure 2.

Figure 2

FGF-binding ability of CP123-1, P3E123-1, and E3CaG-1. A) Binding of labeled FGF-2 to plastic coated with increasing concentrations of TSP-1 or TSP-1 fragment. B) Binding of labeled TSP-1 or fragments, at the indicated concentrations, to immobilized FGF-2. Data are the amount of bound FGF-2, as absorbance (Abs), mean and SD of triplicates. C) Formation of the complex between FGF-2 and the indicated TSP-1 fragment in solution. Samples were analyzed in Western blot. Arrow indicates the putative FGF-2/E3CaG-1 complex. Molecular weight markers (in kDa) are on the left. Experiments in A were done in calcium-free buffers, in B calcium concentration was < 0.1 mM.