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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2007 Oct 9;40(4):700–709. doi: 10.1016/j.biocel.2007.10.002

Figure 3.

Figure 3

Localization of the FGF-binding site in the type III repeats of TSP-1. A) Binding of labeled FGF-2 to plastic coated with increasing concentrations of TSP-1 fragments. Data are the amount of bound FGF-2, as absorbance, mean and SD of triplicates. B) Binding of labeled E3Ca-1 or Ca-1, at the indicated concentrations, to immobilized FGF-2. C) Competition assay: binding of labeled TSP-1, E3Ca-1 and Ca-1 to immobilized FGF-2 in the presence of 2.5 µM unlabeled E3Ca-1, Ca-1, or TSP-1 used as competitors (see legend). Data are the percentage of control binding, in the absence of competitors. D) Effect of the monoclonal anti-TSP-1 antibodies B5.2 and D4.6 (50 µg/ml) on the binding of TSP-1, E3Ca-1, or Ca-1 to immobilized FGF-2. Binding is the percentage of control, in the absence of antibodies. Experiments were done in calcium-free buffers.