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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2007 Oct 9;40(4):700–709. doi: 10.1016/j.biocel.2007.10.002

Figure 4.

Figure 4

Real time biomolecular studies of FGF-2 interaction with TSP-derived peptides. A) Overlay of blank-subtracted sensograms showing the binding of FGF-2 (1400, 1120, 840, 560, 280 and 140 nM from top to bottom) to immobilized E123CaG-1. B) Competition assay. FGF-2 was loaded onto the BIAcore CM5 sensorchip containing immobilized E123CaG-1 in the absence or in the presence of the indicated TSP-1-fragment (1.5 µM). Data are the percentage of FGF-2 bound to the sensorchip at the equilibrium in the absence of any competitor. Experiments were done in calcium-free buffers.