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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2007 Oct 9;40(4):700–709. doi: 10.1016/j.biocel.2007.10.002

Figure 6.

Figure 6

Antiangiogenic activity of the TSP-1-type III repeats. A) Binding of FGF-2 to endothelial cells. BAEC were incubated with labeled FGF-2 in the absence (control, white column) or presence of the indicated recombinant fragment (7.5 µM grey columns, 15 µM striped columns, 30 µM black columns). The amount of cell-bound FGF-2 is expressed as absorbance. B) Endothelial cell proliferation. BAEC were exposed to the indicated concentration of E3Ca-1 (triangles), Ca-1 (squares), or TSP-1 (25 µg/ml, lines) with (black symbols, solid line) or without (white symbols, dashed line) 5 ng/ml FGF-2 and incubated for 3 days. Proliferation is expressed as absorbance. C) FGF-2-induced angiogenesis in the CAM assay. FGF-2 was administered in the absence or presence of E3Ca-1 on day 8 and pictures were taken 4 days later (n=10). Original magnification: 50 x.