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. 2008 May 7;3(5):e2112. doi: 10.1371/journal.pone.0002112

Figure 1. Jurkat T cell upregulate HIV LTR coincident with Casp8p41 expression.

Figure 1

(A) Jurkat T cells were acutely infected with HIV IIIb or mock and three days post infection transfected with RFP. RFP positive cells were gated and p24 content analyzed by intracellular staining with p24 FITC. (B) HIV or mock infected Jurkat T cells were transfected with HIV LTR luciferase or HIV LTRΔTAR luciferase reporter constructs with or without Renilla cotransfection and luciferase measured in the presence or absence of the HIV protease inhibitor Nelfinavir (Nfv). (C) HIV IIIb or mock infected Jurkat T cells were analyzed daily for viability HIV LTRΔTAR luciferase/Renilla activity and (D) Casp8p41 content. (E) Casp8p41 was also measured in Day 3 HIV infected cells in the presence or absence of Nfv. All results representative of three or more experiments.