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. Author manuscript; available in PMC: 2009 Feb 5.
Published in final edited form as: Virology. 2007 Oct 24;371(1):206–215. doi: 10.1016/j.virol.2007.09.041

Fig. 3. Influence of Hsp90 inhibitors on JSRV Env and Akt expression.

Fig. 3

A. Total cell extracts were obtained from 208F-tr cells in the presence of DMSO (negative control) or from 208-tr that had reverted their transformed phenotype in the presence of geldanamycin (GA) or herbimycin A (HA). In addition, cell extracts were also obtained from 208F-tr that returned to the transformed phenotype once GA or HA were removed from the culturing media. 200 μg of cell extracts were immunoprecipitated and analysed by western blotting as indicated below each panel. Note that the JSRV Env expressed in 208F-tr is tagged with a FLAG epitope at its C terminus. TM indicates the transmembrane domain of the JSRV envelope (Env) glycoprotein. B. 208F-tr cells were cultured in serum free media with the addition of 17-DMAG or DMSO as a negative control for 3, 6, 12 and 24 h. At each time point cells were lysed and analysed by western blotting for the presence of Akt and pAkt. Detection of γ-tubulin was used as loading control.