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. 2008 May;131(5):473–481. doi: 10.1085/jgp.200809971

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© 2008 Clausen

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Time course of force decline in rat soleus during 60 Hz indirect stimulation in buffer or in air. Soleus muscles were mounted in force transducers, adjusted to optimal length for measurement of isometric contractions, and equilibrated for 30 min in thermostated chambers containing 8 ml K.R. buffer at 30°C. The nerves were aspirated into suction electrodes allowing indirect stimulation as previously described in detail (Overgaard et al., 1999). All muscles were then stimulated for 60 s at 60 Hz using 1-ms pulses at 5 nA before and after aspiration of the buffer from the incubation chamber. Each curve represents the mean of observations on five muscles incubated in K.R. buffer (filled symbols) compared with contralateral muscles from the same rats mounted in air (open symbols) with vertical bars denoting SEM of values recorded at the times indicated.