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. 2008 May;131(5):455–471. doi: 10.1085/jgp.200709912

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© 2008 Colinas et al.

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Figure 1. — TEA block of transient K⁺ current in rabbit CB chemoreceptor cells. (A) Representative experiment showing the decrease in the peak current amplitude in depolarizing pulses to +40 mV upon application to the bath solution of TEA at concentrations from 10⁻⁷ M to 10⁻¹ M as indicated. Representative traces obtained in control conditions and during application of 10 μM, 100 μM, 1 mM, and 50 mM TEA are depicted in the inset of the figure. (B) TEA dose–response curve obtained with data (mean ± SEM of 9–12 individual determinations) from 12 CB chemoreceptor cells. The continuous line through the data points shows the best fit obtained with a hyperbolic function with three binding sites, with the following equation:
where %Inhibition represents the TEA-sensitive fraction of the current and was calculated as 100 × (I_Control − I_TEA)/I_Control and i = 3. I_Control was obtained by averaging peak current amplitudes before and after TEA application and I_TEA represents the peak current amplitude obtained by averaging several traces in the presence of the corresponding TEA concentration. The relative amplitude of these three components (B1 to B3) and the overall shape of each binding curve (±SEM) is also indicated in the figure. The inset shows the mean ± SEM of the relative amplitude of the three components (B1 to B3) and their corresponding TEA dissociation constants (Kd₁ to Kd₃) obtained from averaging the individual fits of each cell.

graphic file with name M1.gif — TEA block of transient K⁺ current in rabbit CB chemoreceptor cells. (A) Representative experiment showing the decrease in the peak current amplitude in depolarizing pulses to +40 mV upon application to the bath solution of TEA at concentrations from 10⁻⁷ M to 10⁻¹ M as indicated. Representative traces obtained in control conditions and during application of 10 μM, 100 μM, 1 mM, and 50 mM TEA are depicted in the inset of the figure. (B) TEA dose–response curve obtained with data (mean ± SEM of 9–12 individual determinations) from 12 CB chemoreceptor cells. The continuous line through the data points shows the best fit obtained with a hyperbolic function with three binding sites, with the following equation:
where %Inhibition represents the TEA-sensitive fraction of the current and was calculated as 100 × (I_Control − I_TEA)/I_Control and i = 3. I_Control was obtained by averaging peak current amplitudes before and after TEA application and I_TEA represents the peak current amplitude obtained by averaging several traces in the presence of the corresponding TEA concentration. The relative amplitude of these three components (B1 to B3) and the overall shape of each binding curve (±SEM) is also indicated in the figure. The inset shows the mean ± SEM of the relative amplitude of the three components (B1 to B3) and their corresponding TEA dissociation constants (Kd₁ to Kd₃) obtained from averaging the individual fits of each cell.