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The kinetic parameters for PcrA-catalysed DNA unwinding under multiple-turnover condition for 16-bp duplex DNA substrates of different 3′-ssDNA tail lengths, obtained from analyses of the time-courses in Figure 3. (A) Amplitudes. (B) Rates of the fast phase and slow phase. Here the rate for the second slow phase in the case of the 5- and 10-nt tailed substrates was not presented. (C) The initial unwinding rate.
