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. 2008 Feb 14;36(6):1976–1989. doi: 10.1093/nar/gkm1174

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Dependence of DNA unwinding on [ATP] under multiple-turnover conditions. A 5 nM 20-nt-tailed 16-bp duplex DNA was first pre-incubated with excess PcrA helicase (200 nM) in the reaction buffer at 25°C and the unwinding reaction was initiated by adding ATP. (A) Typical kinetic time-courses at different [ATP]. The solid lines were exponential fits of the data. (B) The initial unwinding rate. The line corresponded to a fit of the data to the Hill equation , where n is the Hill coefficient. The fitted values were K_0.5=10.2 ± 0.8 μM and n=1.9 ± 0.3.

Inline graphic — Dependence of DNA unwinding on [ATP] under multiple-turnover conditions. A 5 nM 20-nt-tailed 16-bp duplex DNA was first pre-incubated with excess PcrA helicase (200 nM) in the reaction buffer at 25°C and the unwinding reaction was initiated by adding ATP. (A) Typical kinetic time-courses at different [ATP]. The solid lines were exponential fits of the data. (B) The initial unwinding rate. The line corresponded to a fit of the data to the Hill equation , where n is the Hill coefficient. The fitted values were K_0.5=10.2 ± 0.8 μM and n=1.9 ± 0.3.