Table 1.

Kinetic parameters of Dpo4 for an undamaged D-1 substrate with varying temperature

dNTP	Kd (μM)	kp (s−1)	kp/Kd (μM−1s−1)	Fidelitya	Average Fidelityb
26°C
dATP	8.2 × 102	0.003	4.0 × 10−6	1.9 × 10−4	2.9 × 10−4
dCTP	3.8 × 102	0.003	7.0 × 10−6	3.3 × 10−4
dGTP	1.2 × 103	0.009	7.2 × 10−6	3.4 × 10−4
dTTP	3.0 × 102	6.4	2.1 × 10−2	1
38°C
dATP	9.9 × 102	0.012	1.2 × 10−5	1.5 × 10−4	4.2 × 10−4
dCTP	1.6 × 102	0.012	7.3 × 10−5	9.1 × 10−4
dGTP	1.7 × 102	0.026	1.5 × 10−6	1.9 × 10−4
dTTP	2.2 × 102	17.7	8.0 × 10−2	1
44°C
dATP	3.9 × 102	0.042	1.1 × 10−4	9.2 × 10−4	5.5 × 10−4
dCTP	4.0 × 102	0.021	5.2 × 10−4	4.4 × 10−4
dGTP	1.3 × 103	0.044	3.5 × 10−4	2.9 × 10−4
dTTP	4.8 × 102	59.7	0.12	1
50°C
dATP	5.0 × 102	0.13	2.6 × 10−4	5.8 × 10−4	4.4 × 10−4
dCTP	5.2 × 102	0.064	1.2 × 10−4	2.6 × 10−4
dGTP	6.8 × 102	0.15	2.2 × 10−4	4.8 × 10−4
dTTP	2.6 × 102	118	0.45	1
56°C
dATP	1.2 × 103	0.42	3.6 × 10−4	7.4 × 10−4	7.9 × 10−4
dCTP	2.5 × 102	0.16	6.3 × 10−4	1.3 × 10−3
dGTP	1.5 × 103	0.24	1.6 × 10−4	3.3 × 10−4
dTTP	3.8 × 102	189	0.49	1

^aCalculated as (k_p/K_d)_incorrect/[(k_p/K_d)_correct+(k_p/K_d)_incorrect].

^bCalculated as ∑[(k_p/K_d)_incorrect/[(k_p/K_d)_correct+(k_p/K_d)_incorrect]^{dATP, dCTP, dGTP}]/3.