Figure 2.

Time courses for basic NESA at target concentrations of 400 nM (a) and 2 nM (b), respectively. Each time course consists of three stages: background of molecular beacon, hybridization and nicking reaction. The gap between the stages corresponds to about 15 s during which the sample was taken out of the spectrofluorometer and a new component added. The final concentration of the molecular beacon is 200 nM for both time courses.