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. Author manuscript; available in PMC: 2009 Apr 2.
Published in final edited form as: Mutat Res. 2008 Jan 16;640(1-2):174–179. doi: 10.1016/j.mrfmmm.2008.01.001

Fig. 1. Hypoxia induces global repression of total RNA and mRNA synthesis.

Fig. 1

A) Total RNA (left) and mRNA (right) labeled with tritiated uridine (cpm) during 1 hr synthesis in Hepa 1–6 cells subjected to normoxia or a time-course of 0.2% oxygen. Values, normalized to cell number, represent an average of 5 replicates. Error bars denote SD. *P<0.005. B) Relative total RNA synthesis in three replicates, Hepa 1–6 cells (left) or normal human fibroblasts (right) subjected to indicated time of normoxia, CoCl2 treatment, <0.01% oxygen (hypoxia), or actinomycin D. C) Relative total RNA synthesis in immortalized HIF-1α-null MEFs (left) or HIF-1β mutant hepatoma cells (right) exposed for indicated time as in (B). Values normalized to normoxic controls.