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. Author manuscript; available in PMC: 2009 Apr 2.
Published in final edited form as: Mutat Res. 2008 Jan 16;640(1-2):174–179. doi: 10.1016/j.mrfmmm.2008.01.001

Fig. 3. Nucleosome-occupancy at specific core promoters inversely correlates with transcription activation in response to hypoxia.

Fig. 3

A) Relative levels of Albumin, AFP, EGR1, VEGF, and Albumin expression (hypoxia/normoxia), generated by quantitative RT-PCR, in Hepa 1–6 cells exposed for 48h to normoxia or 0.2% oxygen; each sample normalized to 18S. An average of at least four experiments is shown, and error bars denote SD. B) ChIP of histone H3 in Hepa 1–6 cells after 48h at normoxia or 0.2% oxygen, followed by quantitative PCR of core promoter regions of Albumin, AFP, EGR1, and VEGF. The %-input bound by histone H3, minus background (IgG), in hypoxia relative to normoxia shown. An average of at least three independent experiments shown, each PCR performed in duplicate, with error bars representing SD.