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. Author manuscript; available in PMC: 2009 Mar 26.
Published in final edited form as: Life Sci. 2008 Jan 29;82(13-14):718–727. doi: 10.1016/j.lfs.2008.01.013

Figure 1. Time course of M2 and M4 internalization in JEG-3 cells.

Figure 1

A. JEG-3 cells were transfected with Flag-M2 (white bars) or HA-M4 (black bars) and stimulated for 0, 15 or 30 minutes with 1mM carbachol. B. JEG-3 cells were transfected with Flag-M2 (white bars) or HA-M4 (black bars) and either not stimulated (NS), stimulated for 10 minutes with 1mM carbachol (carb) or stimulated then washed and allowed to recover for 30 minutes (wash). C. HEK293 cells were treated as in B. Cell surface receptors were measured using the binding of [3H]NMS to intact cells as described in ‘Experimental Procedures.’ Data represent the means ± S.E. of two to six independent experiments, each performed in triplicate.