FIG. 2.

Increased mitochondrial mass accompanied mtDNA depletion. The activities of CS, a mitochondrial enzyme of the tricarboxylic acid cycle, MDH, an enzyme with both mitochondrial and cytosolic locations, and PGK, a cytosolic enzyme of the glycolysis pathway, are expressed as nmol·min⁻¹·mg⁻¹ of protein in adipose tissue homogenate; the amounts of subunit 4 of COX (COX4p), a nuclear-DNA-encoded mitochondrial protein, were expressed as the percentages of the amount in one of the control samples; and the steady-state levels of mRNA from the nuclear DNA genes COX4I1 and UCP2 were expressed as cDNA copies, normalized to the β-actin cDNA level. The open circles show values from samples of HIV-infected patients' adipose tissue, and the closed circles show values from control samples. The median value of each group of data is shown as a bar. The asterisks mark statistically significant differences between results for patients' and control samples.