TABLE 1.
Candida spp. used in this study and concentrations of secreted FOH
| Straina | FOH concn [μM]
|
|
|---|---|---|
| 0% FCS | 10% FCS | |
| C. albicans strains | ||
| ATCC 90028 | 13.7 | 1.7 |
| ATCC 24433 | 32.0 | 2.4 |
| ATCC 36801 | 34.6 | 0.5 |
| ATCC 44373 | 16.9 | 0.9 |
| ATCC 44374 | 51.2 | 3.0 |
| ATCC 76615 | 58.5 | 2.4 |
| Y0119b | 42.2 | 2.3 |
| Mean ± SD | 35.6 ± 16.5 | 2.0 ± 0.9 |
| C. glabrata strains | ||
| ATCC 90030 | 0.8 | 0.5 |
| Y3390b | 0.5 | 0.4 |
| RKI 04-0388 | 0.2 | 0.2 |
| RKI 05-0284.01 | 0.4 | 0.3 |
| RKI 05-0445.01 | 0.1 | 0.2 |
| RKI 05-0559.02 | 0.4 | 0.1 |
| RKI 06-0367 | 0.3 | 0.2 |
| Mean ± SD | 0.4 ± 0.2 | 0.3 ± 0.1 |
| C. parapsilosis strains | ||
| Y0501b | 0.3 | 0.4 |
| ATCC 90018 | 1.2 | 0.8 |
| ATCC 22019 | 0.8 | 0.9 |
| RKI 00-0438 | 0.5 | 0.4 |
| RKI 02-0579 | 0.5 | 0.5 |
| RKI 04-0241 | 0.4 | 0.6 |
| RKI 06-0220 | 0.2 | 0.1 |
| Mean ± SD | 0.6 ± 0.3 | 0.6 ± 0.3 |
| C. tropicalis strains | ||
| ATCC 750 | 1.2 | 0.4 |
| ATCC 90874 | 2.2 | 0.4 |
| RKI 03-0108 | 0.3 | 0.1 |
| RKI 04-0326 | 0.4 | 0.8 |
| RKI 05-559.01 | 0.6 | 0.6 |
| RKI 98-0463 | 0.5 | 0.4 |
| RKI 99-0499.02 | 1.2 | 0.4 |
| Mean ± SD | 1.0 ± 0.7 | 0.5 ± 0.02 |
| C. kefyr strains | ||
| Y0601b | 0.8 | 0.7 |
| RKI 01-0709 | 0.7 | 0.1 |
| RKI 95-0329.02 | 0.2 | 1.1 |
| RKI 95-1915.02 | 0.5 | 2.8 |
| RKI 95-2066 | 0.1 | 2.6 |
| RKI 97-0656 | 0.2 | 5.6 |
| RKI 97-0768.02 | 0.5 | 0.2 |
| Mean ± SD | 0.5 ± 0.3 | 1.9 ± 1.8 |
| C. krusei strains | ||
| MB16b | 0.5 | 0.4 |
| ATCC 6258 | 0.7 | 0.8 |
| ATCC 90878 | 0.8 | 0.6 |
| RKI 03-450.02 | 0.5 | 2.5 |
| RKI 04-0167.04 | 0.5 | 0.9 |
| RKI 05-0126 | 0.6 | 0.9 |
| RKI 06-0365 | 0.6 | 0.6 |
| Mean ± SD | 0.6 ± 0.1 | 1.0 ± 0.6 |
| C. guilliermondii strains | ||
| ATCC 90877 | 1.3 | 0.7 |
| RKI 01-0546 | 0.8 | 0.6 |
| RKI 02-0043 | 0.4 | 0.3 |
| RKI 04-0347 | 0.9 | 0.6 |
| RKI 05-0091 | 0.4 | 0.4 |
| RKI 95-1889 | 0.5 | 0.3 |
| RKI 95-2865 | 0.7 | 0.1 |
| Mean ± SD | 0.8 ± 0.3 | 0.5 ± 0.2 |
| C. dubliniensis strains | ||
| CBS 8500 | 17.5 | 2.1 |
| CBS 8501 | 7.8 | 2.9 |
| RKI 01-0170.01 | 6.0 | 0.1 |
| RKI 01-0265 | 12.5 | 2.5 |
| RKI 01-0268.02 | 10.2 | 1.7 |
| RKI 05-0037 | 6.7 | 0.4 |
| RKI 06-0019 | 9.2 | 1.4 |
| Mean ± SD | 8.7 ± 3.8 | 1.5 ± 1.0 |
a
Shown are the strains used and the concentrations of FOH secreted by them when they were cultivated in RPMI 1640 medium with 10% FCS and without FCS (n = 2; agreement of ≤25%) and the mean ± SD of each Candida species, respectively. Strains were obtained from the Centraal Bureau voor Schimmelcultures (CBS), Baarn, The Netherlands; the American Type Culture Collection (ATCC), Manassas, VA; and the Robert Koch Institute (RKI), Berlin, Germany.
b
C. albicans Y0119, C. glabrata Y3390, C. parapsilosis Y0501, C. kefyr Y0601, and C. krusei MB16 were supplied by Pfizer Laboratories, Illertissen, Germany.