TABLE 2.
Activity levels of NRTIs against multicycle HIV-1 replication
| Virus | EC50 value (μM) ± SD ofa:
|
Protocolb | |||
|---|---|---|---|---|---|
| ETV | LVD | AZT | EFV | ||
| HXB2 | 1.030 ± 0.356 | 0.202 ± 0.120 | 0.014 ± 0.006 | 0.0003 ± 0.0001 | 1 |
| RF | 0.839 ± 0.525 | 0.105 ± 0.084 | 0.0029 ± 0.0021 | 0.0003 ± 0.0001 | 1 |
| SF-2 | 0.526 ± 0.120 | 0.183 ± 0.042 | 0.0063 ± 0.0029 | 0.0004 ± 0.0001 | 1 |
| IIIB | 0.081 ± 0.028 | 0.047 ± 0.030 | 0.0012 ± 0.0007 | 0.0001 ± 0.0001 | 1 |
| LAI | 0.071 ± 0.029 | 0.182 ± 0.149 | 0.0038 ± 0.0021 | 0.0002 ± 0.0001 | 1 |
| NL4-3 | 0.085 ± 0.077 | 0.171 ± 0.025 | 0.0049 ± 0.0026 | 0.0003 ± 0.0001 | 1 |
| NL4-3 | 1.4 | ND | ND | ND | 2c |
| NL4-3 | >10 | ND | ND | ND | 2d |
| 93US143e | 1.753 ± 1.074 | 0.850 ± 0.453 | 0.034 ± 0.020 | 0.0007 ± 0.0006 | 1 |
| ASM 034e | 0.109 ± 0.105 | 0.071 ± 0.024 | 0.018 ± 0.010 | 0.0004 ± 0.0001 | 1 |
| 92US076e | 0.062 ± 0.036 | 0.081 ± 0.017 | 0.008 ± 0.002 | 0.0003 ± 0.0002 | 1 |
| ASM 044e | 0.026 ± 0.026 | 0.040 ± 0.042 | 0.016 ± 0.017 | 0.0005 ± 0.0006 | 1 |
a
The EC50 values ± standard deviations (SD) reported for the first six viruses are averages of four experiments using protocol 1 with 1-h preincubations of virus and cells. ND, not done.
b
The EC50 values reported for protocol 2 are averages of two experiments using various virus and cell preincubation times.
c
Time of drug addition at +2-h virus/cell incubation.
d
Time of drug addition +3-h virus/cell incubation.
e
EC50 values for viruses 92US076 and 93US143 are averages of four experiments, and EC50 values for viruses ASM 034 and ASM 044 are averages of three experiments.