TABLE 1.
Peptide PQ C termini detected in MALDI-TOF-TOF
| m/z | Sequence | Proteina | Accession No. | Mass (kDa) | Matched sequence positions |
|---|---|---|---|---|---|
| 971.53 | GPPPPPGKPQ | Basic salivary proline-rich protein 2 | P02812 | 37.3 | 221-230 |
| 990.60 | GPPPQGGRPQ | Salivary acidic proline-rich phosphoprotein 1/2 | P02810 | 17.0 | 148-157 |
| 1,068.66 | GPPPPPPGKPQ | Salivary acidic proline-rich phosphoprotein 1/2 | P02810 | 17.0 | 137-147 |
| 1,104.19 | GPPPQGGNRPQ | Basic proline-rich peptide P-E (IB-9) | P02811 | 6.0 | 29-39 |
| 1,333.77 | GRPQGPPQGQSPQ | Salivary acidic proline-rich phosphoprotein 1/2 | P02810 | 17.0 | 154-166 |
| 1,390.68b | GGRPQGPPQGQSPQ | Salivary acidic proline-rich phosphoprotein 1/2 | P02810 | 17.0 | 153-166 |
| 1,731.89 | GPPQQGGHPPPPQGRPQ | Salivary acidic proline-rich phosphoprotein 1/2 | P02810 | 17.0 | 93-109 |
| 1,957.14 | PQGPPQQGGHPPPPQGRPQ | Salivary acidic proline-rich phosphoprotein 1/2 | P02810 | 17.0 | 91-109 |
| 2,029.28 | GPPPPPGKPQGPPPQGGNKPQ | Basic proline-rich peptide P-E (IB-9) | P02811 | 6.0 | 18-39 |
a
Proteins identified by searching Mascot, a searching algorithm available at the Matrix Science home page. A human database obtained from ftp://ftp.ncbi.nih.gov/genomes/ containing 40,877 sequences was used. Ion scores greater than 70 were considered significant (P ≤ 0.05) (23) for protein identification.
b
A peptide is absent or has a low intensity in the MS spectra of MALDI-TOF-TOF.