FIG. 1.

(A) Chemical structures of the iminosugar (IS) derivatives used in the study. (B) Experimental outline. After a stable infection was established, the cells were cultured for three passages (9 days) in the presence of 1,000 IU/ml IFN and 1 μM RBV. At P3, after the viral RNA levels dropped below the detection limit, the medium was supplemented with one of the iminosugar derivatives. At the end of P8, the samples were split into three sets: set 1 (black line), all drug regimens remained the same; set 2 (cross-hatched line), all drugs were removed; set 3 (gray line), only iminosugars were continued. After P12, samples from set 1, which had been cultured for nine passages in the presence of IFN-RBV and an iminosugar, were split into sample sets 1, 2a, and 3a and treated in the same manner as described above.