FIG. 2.
After eight passages of the various drug treatments, all drugs were either left on (set 1) or removed (set 2) or cells continued to be cultured in the presence of an iminosugar only for a further two passages (set 3). In all cases, for those columns denoted with an iminosugar concentration, the cells had been treated with a triple combination of IFN-RBV and an iminosugar of that concentration for five passages. The numbers of viral RNA copies from supernatants harvested at P9 (left column) and P10 (right column) were measured by real-time RT-PCR and are shown as a percentage of the numbers of copies for the non-drug-treated BVDV-infected control. The data presented are from two independent experiments (experiments E1 and E2). At P9 and P10, the supernatants from the no-drug control in experiment E1 contained 8.71 × 106 and 5.05 ×106 RNA copies/ml, respectively; and at P9 and P10 in experiment E2, the supernatants contained 5.1 ×106 and 5.5 × 106 RNA copies/ml, respectively.