FIG. 1.

Replicon cells treated with a nucleoside inhibitor clear the replicon, while VX-950 or HCV-796 select for resistant colonies. Huh7 cells that stably maintain a GT 1b subgenomic replicon carrying the neoR gene were incubated with a specific HCV inhibitor at 1, 10, and 15 times the EC₅₀ in the presence of G418. After selection for 3 weeks, the remaining cells were fixed and stained with crystal violet. The replicon cells were treated with the nucleoside inhibitor R1479 (A), NM107 (B), or PSI-6130 (C); the nonnucleoside inhibitor HCV-796 (D); or the protease inhibitor VX-950 (E). A representative example of experiments is shown (n = 6 for R1479, n = 4 for NM107 and PSI-6130, n = 7 for VX-950, and n = 10 for HCV-796).